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KMID : 0390220140250020150
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Journal of Clinical Otolaryngology, Head and Neck Surgery
2014 Volume.25 No. 2 p.150 ~ p.155
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Bacterial Extracellular Proteolysis of Cartilage & Temporalis Fascia
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Park No-Seon
Chang Dong-Sik
Cho Chin-Saeng
Park Seung-Gu
Kim Ji-Chan
Lee Ho-Yun
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Abstract
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Background and Objectives：Temporalis fascia and cartilage are well-known materials for the restoration of defective tympanic membrane. Common organisms of postoperative infection in tympanoplasty are S. aureus and P. aeruginosa. This study was done to understand the resistance of cartilage and fascia to extracellular bacterial proteolytic enzyme of the pathogens.
Materials and Methods：S. aureus, P. aeruginosa, E. coli were put in LB broth and Nutrient broth, incubated each other during 24 hours under 37℃. We concentrated culture supernatants on ammonium sulphate precipitation, and dialyzing it with normal saline. Extracellular enzyme with same sized cartilage and temporalis fascia tissue were nourished during 48 hours. The amounts of degradated proteins were measured, then proteins on molecular weight enforced by SDS-PAGE were classified. The activities of proteolytic enzyme by enforcement of zymography were detected.
Results：We showed the proteolytic activities of cartilage and temporalis fascia by the reaction of extracellular enzyme. S. aureus had the most potent destructive power and the cartilage was more resistant to bacterial extracellular proteolysis in S. aureus than fascia.
Conclusions：It could suggest that the cartilage is good for the graft material in aspect of the resistance to extracellular bacterial proteolysis.
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KEYWORD
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Cartilage, Fascia, Tympanoplasty, Proteolysis, Chronic otitis media
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